Abstract:In order to improve the economic value of camellia meal and make full use of the camellia meal resources, tea saponin was extracted from camellia meal by continuous multi-stage countercurrent extraction process using hot water, and the parameters of single-stage extraction and continuous multi-stage countercurrent extraction process were optimized. The results showed that the best conditions for single-stage extraction were as follow:extraction time 85 min, extraction temperature 80℃, material-liquid ratio 1∶16g/mL, and the most suitable extraction size of camellia meal 60-100mesh. On the basis of single-stage extraction, the optimal number of extraction stages for continuous multi-stage countercurrent extraction was determined to be 3 stages, and the optimum extraction time for each stage was 20min. Under those conditions,the content of tea saponin in the extract was 33.14mg/mL, which was 123% higher than that of single-stage extraction. Extraction time was shortened 29.41% and extraction efficiency was improved at the same time. In this study, an aqueous solution combined with continuous multi-stage countercurrent extraction was used to obtain tea saponin with a higher mass concentration, and the extraction time was shortened. The extraction process was green and environmentally friendly, and industrial production was easier to achieve.