In order to investigate the effect of different transportation time on the physiology and quality of mango during the later low-temperature storage, simulated transportation experiments of mango on the analog transport vibration table at 180r/min for 12,24 and 36 h were carried out. The changes in moisture distribution, free moisture content, weight loss rate, titratable acid content, vitamin C content, respiration intensity, pectinmethylesterase (PME) content, and polygalacturonase (PG) content of mango during 20days storage under 13℃ were measured. The results showed that transportation vibration led to different degrees of bruising on mango, accelerated water loss, significantly increased the rate of water loss and respiratory peak, maintained the PME and PG content at a higher level, and lost more titratable acids and vitamin C. At the same time, the impact on the physiological quality of mango was enlarged with the increasing transportation vibration time. Therefore, vibration during transportation process accelerated mango metabolism and the quality declined during the later low-temperature storage. The quality damage of mango in transportation groups of 24h and 12h was acceptable. However, more irreversible damage to mango in transportation group of 36 h was occurred.