In vitro model experiment of α-glucosidase inhibitor was used to screen out 4 kinds of active material, and the optimization design of the mixture formula was carried out. The optimal proportions of formula were Cyclocarya paliurus leaves extract 26.05%, grape skin extract 10.00%, mulberry leaves extract 22.13%, and loquat leaves extract 41.82%.The insulin resistant HepG2 cell model was induced by the high concentration glucose. The results showed that the formula could significantly promote the glucose utilization of the insulin resistant HepG2 cell to improve the insulin resistance, and the effect was concentration-dependent.