Based on the advantageous functions of short chain fatty acids to human intestine and body, the species and content of short chain fatty acids(SCFA) produced from Chinese yam oligosaccharides by lactic acid bacteria and bifidobacteria fermented in simulated intestinal environment were investigated, respectively. The results showed that Chinese yam oligosaccharides could be utilized as carbon source by lactic acid bacteria and bifidobacteria in simulated colonic fermentation environment. SCFA, such as lactic acid, acetic acid and propionic acid with positive effects on the body were produced in the process. And the concentration of SCFA is related to strains of Lactobacillus and fermentation time. The lactic acid concentration in 48h fermented liquid of Lactobacillus plantarum reached (13.800±0.243)mg/mL. The acetic acid concentration in 48h fermented liquid of Bifidobacterium animalis reached (1.850±0.003)mg/mL; while the propionic acid concentration in 8h fermented liquid of Bifidobacterium animalis reached (0.082±0.001)mg/mL. The results suggested that SCFA could be produced by intestinal probiotics with the adjustment of diet structure to keep our intestine and body healthy.