Zearalenone (ZEN) is a kind of fungal toxin produced mainly produced by Fusarium, which can easily contaminate food crops. Using the specificity of biological enzyme to destroy the molecular structure of ZEN is an effective method to eliminate ZEN pollution. Through screening, we found a microbial strain that can efficiently degrade ZEN, and the whole genome sequence information of the strain was obtained by the genome sequencing. The sequence alignment of ZEN-degrading enzyme ZHD101 was then performed, and the homologous sequence ZHD795 was obtained. To determine its ZEN-degrading activity, the gene fragment was synthesized chemically and cloned into the E.coli expression system, and the protein was then purified using affinity chromatography. The degradation activity was tested and the HPLC results showed that ZHD795 could degrade ZEN molecule efficiently. At the same time, the level of ZEN degradation activity of ZHD795 and ZHD101 was compared. Under the same conditions, the degradation activity of ZHD795 to ZEN was 2.5 times than that of ZHD101. This research showed that a highly active ZEN degradation enzyme with good application prospect was obtained.