A method for determination of benzopyrene (BaP) in vegetable oil by reversed phase high performance liquid chromatography (RP-HPLC) was established. Vegetable oil was dissolved in the mixed liquor of tetrahydrofuran and acetonitrile, then sample liquid was injected into HPLC for testing. BaP was separated by C18 colum, and quantified by fluorescence detector. The excitation wavelength was 384nm, and the emission wavelength was 406nm. Mobile phase was acetonitrile and 1‰ acetic water (88∶12), and the flow rate was 1.0mL/min. The retention time was applied for the qualitative analysis with BaP as the external standard. The method repeatability limit(r) 0.81 and reproducibility limit(R) 0.84 were more than twice the determination results of absolute difference value of 0.1. Meanwhile, the recovery rate was above 90%, and the linear range was from 0.5ng/mL to 100ng/mL. The method detection limit was 0.09μg/kg. This method was simple and low cost, which was suitable for the rapid determination of benzopyrene in vegetable oil.