MOF-5 was prepared by a direct synthesis method. Effects of MOF-5 mass, curcumin concentration, adsorption temperature and and adsorption time were investigated by the single-factor experiment. Based on the results, the orthogonal test method was used to optimize the adsorption conditions. The optimal adsorption rate of the complex was simulated in vitro, and the bioavailability of the complex was studied. The best adsorption conditions were as follows:MOF-5 quality 0.4g, curcumin concentration 0.20mg/mL, adsorption temperature 30℃, and adsorption time 36min. Under the optimal conditions, the best adsorption rate was 94.57%. After the simulated gastrointestinal digestion, the average release rate of curcumin in gastric juice was 48.93%. After passing through the digestive tract, the average content of curcumin was 10.32%. Curcumin absorbed by MOF-5 was released in stomach and absorbed in intestine.