In this study, a method for simultaneous determination of benzoic acid, sorbic acid, saccharin sodium and acesulfame in food by HPLC was established. The samples were extracted by water, precipitated protein by potassium ferrocyanide and zinc acetate, adjusted pH with ammonia water and centrifuged. Agilent ZORBAX SB-Aq column was applied with mobile phase consisting of methanol and 0.02mol/L ammonium acetate solution by gradient elution with a flow rate of 0.7mL/min. Targets were detected by diode array detector detection with detection wavelength of 230nm and a double qualitative determination of retention time and spectra was performed. The spectral scanning range was 200~400nm. Benzoic acid, sorbic acid, saccharin sodium, and acesulfame K could be effectively separated within 14min. A good linear relationship was obtained in the range of 1-100μg/mL. The linear correlation coefficient was greater than 0.9998. The average recoveries ranged from 88.5% to 98.4% and the precision less than 5%. The detection limits ranged from 1mg/kg to 2mg/kg. The method has wide application range, high sensitivity, and good accuracy, and is suitable for the detection of benzoic acid, sorbic acid, saccharin sodium, and acesulfame in various foods.