Melanin is produced by plants or micro-organisms, and has broad application prospects in the food, cosmetics and medicine. Bacteria S_8nyzx-1 that can produce melanin was isolated from black garlic of 96h fermented. The strain was identified according to colony morphology and molecular biology. The structure characteristic of this melanin was identified using the techniques of UV and IR spectroscopy,and high temperature ability for a short time was determined. The results showed the colony was neat edges, white opaque, dry surface, wrinkled, and surrounded by a small amount of brown pigment on LB. 16S rDNA fragment (1419 bp of size) was 99.8% of similarity with Bacillus subtilis. The strain had a high ability of survive 10min at 93℃ water bath. The melanin had strong absorption peak at 370-390nm through UV and with the indole structure through IR, which was considered for eumelanin according the characteristics of the bacteria can use L-tyrosine.