To investigate the pollution of organophosphorus pesticides in fresh tea leaves, a simple and rapid analytical method was established for simultaneous determination of seven organophosphorus pesticides (dichlorvos, dimethoate, chlorpyrifos, methyl parathion, malathion, fenitrothion, and triazophos) in fresh tea leaves by ultrasound assisted extraction and dispersive solid phase extraction (DSPE) coupled with gas chromatography. The samples were ultrasonically extracted with acetonitrile (containing 1% acetic acid). Subsequently, the extract was cleaned-up by using primary secondary amine, octadecylsilane bonded silica and graphitized carbon black as adsorbents according to the method of DSPE. Then, the residues were determined by a heated DB-1701 capillary column with flame photometric detector, and quantified using the matrix-matched external standard method. Good linearities between concentrations and peak areas were achieved in the concentration range of 0.01-4.00mg·L-1 for target pesticides, with correlation coefficients higher than 0.998. The average recoveries of target pesticides in fresh tea leaves were 77.7%-98.2% with relative standard deviations (n=5) ranging from 2.4% to 8.7%. The limits of detection for target organophosphorus pesticides were 0.0034-0.0136mg·kg-1 and the limits of quantification were 0.012-0.046mg·kg-1. The method was simple, accurate, solvent saving and without special instrument making it suitable for simultaneous determination of seven organophosphorus pesticides in fresh tea leaves.