Composition of flavonoid glycosides in corn silk were analyzed in this study. Ethyl acetate extractive of corn silk was purified by the silica gel column and sephadex LH-20 column chromatography. Flavonoid glycosides in corn silk were analyzed by HPLC-MS. Results showed that ethyl acetate extractive of corn silk had three kinds of flavonoid glycosides, which were 5,7,4′-trihydroxy -3′-methoxyflavone-6-C-2″-O-dirhamnoside, 5,7,4′-trihydroxy-3′-methoxyflavone-3-C-arabinose-6-C-rhamnoside, and ax-5″-methane-3′-methoxymaysin were analyzed by HPLC-MS.