The effects of individual treatments with gas impingement on fruit quality and reactive oxygen species metabolism of strawberries were analyzed by one-way analysis of variance, principal component analysis (PCA), partial least squares regression (PLSR), and path analysis (PA). One-way analysis of variance showed that ozone significantly inhibited the strawberry fruit rot index and respiration intensity at room temperature, while suppressed decay index as well as the methane dicarboxylic aldehyde (MDA)content during storage at 1℃. Nitrogen inhibited the decay index and MDA content of strawberries stored at 20℃.It was supported by PCA results which concluded that the ozone treatment was helpful for strawberries storage. PLSR indicated that decay index was positively correlated with respiration intensity, anthocyanins, LOX, and SOD activity, but negatively correlated with CAT, radical scavenging ability of hydroxyl, and ultra oxygen anion. Hydrogen peroxide was positively correlated with POD and PPO. It was showed by PA that respiratory intensity, DPPH radical scavenging ability, hydroxyl radical scavenging ability, and APX were all direct factors for strawberry decay index, while total phenol, reducing power and PPO had negative indirect effects on decay index. GSH, SOD and POD directly impacted hydrogen peroxide of strawberry fruits, but PPO, APX, DPPH radical scavenging ability, and ultra oxygen anion removal ability were indirect factors of hydrogen peroxide.