A rapid method for determination of the neotame content in Laiwu ginger-liquor by HPLC was developed. Neotame was qualitatively analyzed on a Symmertry Shield RP18 column. The mobile phase was acetonitrile 0.02mol/L(NH₄)₂HPO₄(30∶70,V/V). The column temperature was 30℃ and the UV detector (218nm) was used. The separation was achieved within 8min. The standard working curve of neotame had a good liner relation in the range from 5.0mg/L to 100.0 mg/L (R=0.9999). The relative standard deviation was 0.62% (n=6) and the average recovery was 98.7%. Meanwhile, the detection limit was 2.5×10-4g/kg.The method was simple and fast and could be used to determine the neotame content in Laiwu ginger-liquor.