Direct drinking vinegar, especially Fruit vinegar production is one of the most important way for deep processing of agricultural byproducts. This article is based on acetic acid bacteria was cultured under the conditions with different temperature, rotation rate, alcohol concentration, and inoculation. The total acid content was determined, and the growth of bacteria on the semisolid culture medium with pear slag was observed. Finally, response surface analysis was used to optimize the growth condition of acetic acid bacteria, and the optimum conditions were found as follows: rotation rate was 136r·min-1, temperature was 32℃, the weight of the pear slag was 28%, alcohol content was 3.6%, inoculation was 4.0%. The result could provide a theory reference and practice basis for industrial production of the pear vinegar.