The probiotic properties of two self-chosen lactobacillus strains were studied to provide the theorerical foundation for the research and development of lactic acid fermentation beverage. The stress resistance of lactobacillus plantarum R23 and lactobacillus casei R35 were investigated in a simulated enviroment of gastric acidity and bile salts, the adhesion ability were determined such as their aggregation properties and cell-surface hydrophobicity. The antioxidation of the broth including the scavenging effects on superoxide radical and hydroxyl radical was evaluated in vitro. The results showed that the stress resistance of the both strains was superior to the control group lactobacillus delbrueckii subsp 6045. The tolerance of pH value was 2.5 and bile salt was 5g·L-1 for the lactobacillus plantarum R23, showing the strongest stress resistance. The adhesion of the lactobacillus casei R35 was the strongest in contrast with the control group, the hydrophobicity of the lactobacillus paltarum R23 was stronger, while its aggregation properties was weaker. The extracellular products of three strains had antioxidant capacity after 24h culture, while lactobacillus paltarum R23 showed the best. Its scavenging rate to superoxide radical and hydroxyl radical was 77.8% and 80.94% respectively, which was 36.72% and 5.81% higher than the control group.