The microwave and ultrasonic techniques were used to extract Gypenosides from Gynostemma pentaphyllum (Thunb.) makino. The optimum extraction conditions were investigated by the single facter and orthogonal design. The optimal conditions for the microwave extraction were the ratio of material to solvent of 1∶25 (g/mL), extracting time of 11 min , microwave power of 400W. Under the optimal conditions, the rate of Gypenosides was 7.59%. The optimal conditions for the ultrasonic extraction were as follows: the ratio of material to solvent of 1∶25 (g/mL), extracting temperature of 70℃, extracting time of 20 min, ultrasonic power of 400W. Under the optimal conditions, the rate of Gypenosides was up to 8.01%.