The microwave-assisted extraction technology was used to increase the extraction ratio of polysaccharides from the root of Agaricus bisporus stipe. The influence of factors on the extraction of the polysaccharides was studied by single factor test and orthogonal experiment design methods, including microwave power, lid-liquid ratio, radiation time and extraction times. Results showed that the optimum conditions were obtained as follows: the microwave power 60%, the ratio of material to solvent 1∶12, the time for microwave extraction 6 minute and extraction times 4. Under the technological conditions, the content of polysaccharides was 1.64%.