Using the fiber of Carboxen/PDMS, influences of sample temperature, adsorption time on the number and amount of volatiles identified from lentinus edodes when analyzed by solid phase microextraction (SPME) and GC-MS were investigated. The preferred SPME conditions found were sampling at 60℃ for 45min in which total of forty-three volatiles were identified. The volatiles of great contribution to lentinus edodes flavor included seven oxygen-containing heterocyclics, nine sulfur-containing compounds, five aldehydes, six alcohols and three ketones, where dimethyl dithioether(4.36%), dimethyl trithioether(13.36%),1,2,4-trithiolane(3.70%),1-octen-3-ol(1.11%) were in high amount.