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Manipulating redox and ATP balancing for improved pro鄄

Progress for Bio鄄Refinery of Malic Acid

*
JIANG Shaotong,摇 LI Xingjiang
(Key Laboratory for Agricultural Products Processing of Anhui Province/ School of Food and Biological

Engineering, Hefei University of Technology, Hefei 230009, China)

Abstract: Being a top bio鄄derived value added chemical, malic acid is used widely in various industries
including chemical engineering, food, and pharmaceutical. It is of great significance to produce malic
acid by converting sustainable cellulosic raw materials. This review has introduced and summarized the
malic acid bio鄄refinery process, which was inoculating the fermented raw materials, analyzing the main
metabolic pathways of the fermented raw materials, identifying the key enzymes for malic acid produc鄄
tion, and determining the malic acid metabolism improvement strategy. At the same time as the technical
summary, the correlation between the internal metabolism of the transformed organism and the external
conditions was analyzed, and the forward trend of malic acid biotransformation was proposed. The forward
trend was simultaneous of saccharification and fermentation processes to improve transformation efficiency,
improving and enhancing cell tolerances and fermentation adaptabilities, promoting co鄄fermentation of
five鄄carbon sugars and six鄄carbon sugars, decreasing greenhouse effect and controlling energy balance.
This review was expected to provide meaningful insight to the development of the malic acid bio鄄refinery
industry.

Keywords: malic acid; biomass sugars; sour agents; valued chemicals; bio鄄refinery

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