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contents [ J ]. Metabolic Engineering, 2015, 29: duction of succinate in E. coli[J]. Metabolic Engineer鄄
46 - 55. ing, 2011, 13(1):76 - 81.
[45]摇 ABBOTT D A, ZELLE R M, PRONK J T, et al. Meta鄄 [47]摇 LIANG L, LIU R, WANG G, et al. Regulation of NAD
+
bolic engineering of Saccharomyces cerevisiae for produc鄄 (H) pool and NADH/ NAD ratio by overexpression of
tion of carboxylic acids: current status and challenges nicotinic acid phosphoribosyl transferase for succinic
[J]. Fems Yeast Research, 2010, 9(8):1123 - 1136. acid production in Escherichia coli NZN111[J]. Enzyme
[46] 摇 SINGH A, SOH K C, HATZIMANIKATIS V, et al. & Microbial Technology, 2012, 51(5):286 - 293.
Manipulating redox and ATP balancing for improved pro鄄
Progress for Bio鄄Refinery of Malic Acid
*
JIANG Shaotong,摇 LI Xingjiang
(Key Laboratory for Agricultural Products Processing of Anhui Province/ School of Food and Biological
Engineering, Hefei University of Technology, Hefei 230009, China)
Abstract: Being a top bio鄄derived value added chemical, malic acid is used widely in various industries
including chemical engineering, food, and pharmaceutical. It is of great significance to produce malic
acid by converting sustainable cellulosic raw materials. This review has introduced and summarized the
malic acid bio鄄refinery process, which was inoculating the fermented raw materials, analyzing the main
metabolic pathways of the fermented raw materials, identifying the key enzymes for malic acid produc鄄
tion, and determining the malic acid metabolism improvement strategy. At the same time as the technical
summary, the correlation between the internal metabolism of the transformed organism and the external
conditions was analyzed, and the forward trend of malic acid biotransformation was proposed. The forward
trend was simultaneous of saccharification and fermentation processes to improve transformation efficiency,
improving and enhancing cell tolerances and fermentation adaptabilities, promoting co鄄fermentation of
five鄄carbon sugars and six鄄carbon sugars, decreasing greenhouse effect and controlling energy balance.
This review was expected to provide meaningful insight to the development of the malic acid bio鄄refinery
industry.
Keywords: malic acid; biomass sugars; sour agents; valued chemicals; bio鄄refinery
(责任编辑:叶红波)