An ultra performance liquid chromatography-tandem mass spectrometry (UPLC- MS /MS) method for the determination of five carbamate pesticides in spinach was established. Samples were homogenized and extracted by ultrasonic with acetonitrile-water(50∶50 in volume ratio), and then salted out and purified with primary secondary amine sorbent. Under the electrospray ionization (ESI+mode), data of the target parent ions and product ions were collected with mass spectrometric multi-response monitoring scanning detection. The matrix calibration curves of five carbamate pesticides showed the good linearity within the concentrations of 0.1-20.0μg/L with the good correlation coefficient (r＞0.9991). The average recoveries of three spiked levels ranged from 91.0%-101.0%, while the limits of detection and limits of quantitation were 0.10-0.20μg/kg and 0.25-0.50μg/kg, respectively. The relative standard deviations were 2.15%-5.57%. This method has good sensitivity and high precision, which is suitable for practical determination of five carbamate pesticides in spinach.