(1.沈阳农业大学 食品学院, 辽宁 沈阳 110866;2.辽宁省食品发酵技术工程研究中心, 辽宁 沈阳 110866;3.宁夏食品微生物应用技术与安全控制重点实验室/宁夏大学 食品与葡萄酒学院, 宁夏 银川 750021;4.沈阳市微生物发酵技术创新重点实验室, 辽宁 沈阳 110866;5.宁夏伊品生物科技股份有限公司, 宁夏 银川 750001)
(1.Food Science College, Shenyang Agricultural University, Shenyang 110866, China;2.Engineering Research Center of Food Fermentation Technology, Shenyang 110866, China;3.Ningxia Key Laboratory of Food Microbiology Application Technology and Safety Control/College of Food and Wine, Ningxia University, Yinchuan 750021, China;4.Shenyang Key Laboratory of Microbial Fermentation Technology Innovation, Shenyang 110866, China;5.Ningxia Yipin Biotechnology Co Ltd, Yinchuan 750001, China)
为了解决益生菌在贮藏和摄食过程中的活性损失,及2种以上益生菌混合使用可能出现的竞争和拮抗等问题,更好地发挥双菌或多菌分步释放、协同增效作用,以青春双歧杆菌FS2-3(B.adolescens FS2-3)为内芯微球,枯草芽孢杆菌SN15-2(B.subtilis SN15-2)为外壳微球,对2种益生菌进行分隔包埋。采用海藻酸钠为壁材,同时添加巯基化羧甲基纤维素钠(CMC-SH)加强益生菌在肠道内的黏附性,利用内源乳化法和挤压法制备益生菌双菌多核微胶囊。将微胶囊的包埋率作为评价指标,通过单因素实验和响应面试验确定益生菌双菌微胶囊制备的优化条件。结果表明,当内芯海藻酸钠质量分数为1.82%,CMC-SH在菌液中的质量分数为0.9%,水相油相体积比为 1.0∶2.9, 外层海藻酸钠质量分数为2.9%时,益生菌双菌微胶囊的包埋率可达72.93%。该工艺条件下的益生菌双菌微胶囊具有良好的耐酸性和肠溶性。在人工肠液中,微胶囊180min时释放率达到86.3%,基本完全崩解,达到了理想效果。研究旨在为混合益生菌微生态制剂普遍存在的活性保持与协同作用技术开发提供参考。
In order to solve the loss of activity of probiotics in the process of storage and feeding, as well as the competition and antagonism that may occur when two or more probiotics are mixed, and to better exert the step-by-step release and synergistic effect of two or more bacteria, Bifidobacterium adolescentis FS2-3 as the inner core microsphere and Bacillus subtilis SN15-2 as the shell microsphere were used to separate and embe the two probiotics. Sodium alginate was used as wall material and sodium mercapto carboxymethyl cellulose (CMC-SH) was added to enhance the adhesion of probiotics in intestinal tract. Probiotics double bacteria multi-core microcapsules were prepared by endogenous emulsification and extrusion. The microcapsule embedding rate was used as the evaluation index, and the optimal conditions for the preparation of probiotic double bacteria microcapsules were determined by single factor and response surface experiments. The results showed that when the concentration of sodium alginate in the inner core was 1.82%, the concentration of CMC-SH in the bacterial solution was 0.9%, the volume ratio of water to oil was 1.0∶2.9, and the concentration of sodium alginate in the outer layer was 2.9%, the embedding rate of probiotics double bacteria microcapsules could reach 72.93%. The probiotic double bacteria microcapsules under this process condition had good acid resistance and intestinal solubility. In the artificial intestinal fluid, the release rate of microcapsules reached 86.3% at 180min, which was basically completely disintegrated, and the ideal effect was achieved. The results of this study provided a reference for solving the technical problems of activity maintenance and synergism of mixed probiotic microecologics in China.