群体感应信号分子AI-2调控乳酸菌生物膜形成机制的研究进展
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(云南农业大学 食品科学技术学院, 云南 昆明 650201)

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云南省教育厅项目(2022Y258);国家自然科学基金资助项目(32260578);云岭产业技术领军人才项目[云发改人事(2014)1782号]。


Research Progress of Formation Mechanism of Quorum Sensing Signal Molecule AI-2 Regulated Lactic Acid Bacteria Biofilm
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(College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China)

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Project of Yunnan Provincial Department of Education (2022Y258);National Natural Science Foundation of China(32260578);Yunling IndustrialTechnology Leading Talent Project[Yunnan Development and Reform &Human Affairs(2014)No.1782].

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    摘要:

    乳酸菌生物膜具有黏附性、抗逆性以及抗菌活性等多种物理特性和生理功能,被广泛应用于改善食品质构以及食品的生物保鲜中。乳酸菌生物膜的形成需经历附着、形成、成熟、老化脱落和重新附着5个阶段,其形成受到群体感应系统的调控。群体感应系统(quorum sensing system,QS)是细菌中广泛存在的一种基因表达调控系统,该系统通过信号分子靶向调控相关基因的表达,从而调控细菌的生理功能。LuxS/AI-2型QS是调控乳酸菌益生特性的主要群体感应系统。明悉乳酸菌的LuxS/AI-2型QS及其调控生物膜形成的机制,对于乳酸菌在食品工业中的进一步应用至关重要。重点介绍了乳酸菌生物膜的形成过程,以及LuxS/AI-2型QS调控乳酸菌生物膜形成的5个调控元件,即信号分子AI-2(autoinducer-2)、关键调控基因(luxS、tuf、fba、gap)、关键调控蛋白(LuxS、LacI、AraC、PadR以及Rbs家族蛋白)、信号分子AI-2的可能受体蛋白(LuxP、LsrB、RbsB和含有dCACHE结构域的受体蛋白)以及关键代谢路径的最新研究进展;总结了信号分子AI-2调控乳酸菌生物膜形成的可能分子机制,以及信号分子AI-2受体蛋白的筛选策略。希望为深入了解LuxS/AI-2型QS调控乳酸菌生物膜的形成提供理论指导。

    Abstract:

    Lactic acid bacteria biofilm has various physical properties and physiological functions such as adhesion, resistance and antibacterial activity. It is widely used in improving the texture of food and in the biological preservation of food. The formation of lactic acid bacteria biofilm needs five stages as attachment, formation, maturation, aging off and reattachment, and is regulated by the quorum sensing system. Quorum sensing system (QS) is a widespread gene expression regulatory system in bacteria, which regulates the physiological functions of bacteria by targeting the expression of related genes through signaling molecules. The LuxS/AI-2 type QS is the main QS for regulating the probiotic properties of lactic acid bacteria. Understanding the LuxS/AI-2 type QS of lactic acid bacteria and its mechanism of regulating biofilm formation is crucial for further applications of lactic acid bacteria in the food industry. The recent research progress on the formation processes of lactic acid bacteria biofilms, and the five regulatory elements of LuxS/AI-2 type QS regulating lactic acid bacteria biofilm formation, including the signaling molecule AI-2 (autoinducer-2), key regulatory genes (luxS, tuf, fba, gap), key regulatory proteins (LuxS, LacI, AraC, PadR, and Rbs family proteins), the possible receptor proteins of signaling molecule AI-2 (LuxP, LsrB, RbsB and the dCACHE domains receptor proteins) and key metabolic pathways was highlighted. Furthermore, the possible molecular mechanisms of signal molecule AI-2 regulating biofilm formation of lactic acid bacteria, as well as the screening strategy of signal molecule AI-2 receptor protein were summarized. The aim of study was to provide a theoretical guide to gain insight into the LuxS/AI-2 type QS that regulates the biofilm formation of lactic acid bacteria.

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魏光强,王藤,赵波,陶继芳,王道滇,黄艾祥.群体感应信号分子AI-2调控乳酸菌生物膜形成机制的研究进展[J].食品科学技术学报,2023,41(2):141-153.

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  • 收稿日期:2022-09-30
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  • 在线发布日期: 2023-04-13
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