4种杂豆多糖对乙醇诱导小鼠急性胃黏膜损伤的辅助保护作用
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(1.南昌大学 食品科学与技术国家重点实验室/中国-加拿大食品科学与技术联合实验室(南昌)/ 江西省生物活性多糖重点实验室, 南昌 330047;2.加拿大农业及农业食品部 圭尔夫研究与发展中心, 加拿大 安大略 圭尔夫 N1G5C9)

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国家重点研发计划项目(2018YFE0108300)。


Protective Effects of Four Legume Polysaccharides on Ethanol-Induced Acute Gastric Mucosal Lesions in Mice
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Affiliation:

(1.State Key Laboratory of Food Science and Technology/China-Canada Joint Laboratory of Food Science and Technology (Nanchang)/Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang 330047, China;2.Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph N1G5C9, Canada)

Fund Project:

National Key Research and Development Program Project of China (2018YFE0108300).

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    摘要:

    胃溃疡是因胃黏膜损伤而导致的消化性溃疡疾病,因其化学治疗药物存在各种副作用,寻求更安全有效的天然活性化合物治疗胃溃疡显得至关重要。采用水提醇沉法分别从小黑豆、豇豆、豌豆和鹰嘴豆提取多糖,测定其基本营养组成、单糖组成和分子质量分布,探究4种杂豆多糖对乙醇诱导的小鼠急性胃黏膜损伤的保护作用及其潜在机制。研究结果表明:小黑豆、豇豆、豌豆和鹰嘴豆多糖的糖含量分别为66.40%、71.79%、67.41%、53.31%,分子质量分布较宽;豇豆、豌豆及鹰嘴豆多糖的单糖组成主要为阿拉伯糖、半乳糖和半乳糖醛酸,属果胶类酸性多糖,而小黑豆多糖主要由半乳糖和甘露糖组成,属中性多糖。小黑豆、豇豆和鹰嘴豆多糖的胃黏膜损伤抑制率分别为37.1%、34.4%和29.8%,均可减轻小鼠胃黏膜损伤状况,其中豇豆多糖可显著抑制白细胞介素6和肿瘤坏死因子α表达(P<0.05),小黑豆多糖能显著降低胃组织中丙二醛含量及提高总超氧化物歧化酶活性和还原型谷胱甘肽含量(P<0.05),二者具有更好的胃黏膜保护效果。研究旨在为利用杂豆多糖开发具有护胃功能的健康食品提供理论参考。

    Abstract:

    Gastric ulcer is a peptic ulcer disease caused by gastric mucosal lesion. Because of its various side effects of chemotherapy drugs, it is very important to seek safer and more effective natural active compounds to treat gastric ulcer. Polysaccharides were extracted from small black soybean, cowpea, pea and chickpea by water extraction and alcohol precipitation. The basic nutritional composition, monosaccharide composition and molecular weight distribution were determined, and the protective effects and potential mechanism of four legume polysaccharides on ethanol-induced acute gastric mucosal lesion in mice were explored. The results showed that the sugar contents of polysaccharides from small black soybean, cowpea, pea and chickpea were 66.40%, 71.79%, 67.41% and 53.31%, respectively, and the molecular weight distribution was wide. The monosaccharide composition of cowpea, pea and chickpea polysaccharides were mainly arabinose, galactose and galacturonic acid, which belonged to pectin acidic polysaccharide, while the small black soybean polysaccharides were mainly composed of galactose and mannose, which belonged to neutral polysaccharide. The inhibition rates of small black soybean, cowpea and chickpea polysaccharides on gastric mucosal lesion were 37.1%, 34.4% and 29.8%, respectively, which could reduce the gastric mucosal lesion in mice. Cowpea polysaccharides could significantly inhibit the expression of interleukin-6 and tumor necrosis factor-α (P < 0.05). Small black soybean polysaccharides could significantly reduce the content of malondialdehyde in gastric tissue and increase the activity of total superoxide dismutase and the content of reduced glutathione (P < 0.05). Both two polysaccharides had better gastric mucosal protective effect. This study wished to provide a theoretical reference for the development of healthy food with gastric protection function by using legume polysaccharides.

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黄钻元,胡梦伟,王瑞,罗宸东,宋萧萧,崔武卫,殷军艺.4种杂豆多糖对乙醇诱导小鼠急性胃黏膜损伤的辅助保护作用[J].食品科学技术学报,2023,41(2):81-91.

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  • 收稿日期:2022-06-25
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  • 在线发布日期: 2023-04-13
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