黑豆β-伴大豆球蛋白和大豆球蛋白与矢车菊素-3-O-葡萄糖苷相互作用的光谱特征及分子对接研究
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河南工业大学粮油食品学院

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TS214.2

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国家自然科学(No. 31972003);河南省高校科技创新团队项目(No. 20IRTSTHN023)。


Interaction of β-conglycinin and glycinin with cyanidin-3-O-glucoside: spectroscopic characteristics and molecular docking study
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School of Food Science and Technology, Henan University of Technology

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    摘要:

    矢车菊素-3-O-葡萄糖苷(C3G)在不同pH值环境中的主要存在形式不同,其抗氧化能力也有所差异。黑豆蛋白作为一种常见的膳食蛋白,具有作为不稳定生物活性化合物载体的潜力,了解黑豆蛋白与C3G的相互作用机制有利于它们在食品体系中的应用。通过多种光谱学分析和分子对接实验研究了在pH值为 2.0、5.0和7.0条件下黑豆β-伴大豆球蛋白(7S)和大豆球蛋白(11S)与C3G的相互作用及其对C3G氧化稳定性和抗氧化能力的影响。研究结果表明,在pH值为 2.0和5.0条件下,C3G与7S和11S结合不仅导致Tyr残基周围的疏水环境减少,极性增加,而且使7S和11S中α-螺旋增加,β-折叠比例下降。在pH 值为7.0条件下,C3G的存在使7S和11S中α-螺旋和β-折叠的百分比呈增加趋势,但β-转角比例降低。此外,C3G与7S和11S结合是一个放热过程,在不同pH值条件下,C3G与7S和11S主要通过氢键和范德华力相互作用使7S和11S静态荧光猝灭。7S和11S均在pH值为 7.0条件下对C3G亲和力最强。分子对接结果表明,7S上的GLU229、ARG356和PRO101残基,11S上的ARG161、VAL162、ILE171和THR176残基在与C3G结合中起关键作用。在不同pH值条件下,7S、11S与C3G结合后均显著增强了C3G的氧化稳定性和抗氧化能力。

    Abstract:

    Cyanidin-3-O-glucoside (C3G) exists in different forms in various pH environments, leading to differences in its antioxidant capacity.Black bean protein,a common dietary protein, has the potential to serve as a carrier for unstable bioactive compounds.Understanding the interaction mechanism between black bean protein and C3G is beneficial for their application in food systems.Interactions between β-conglycinin(7S)and glycinin(11S)soybean proteins with C3G and their effects on the oxidative stability and antioxidant capacity of C3G at pH 2.0,5.0,and 7.0 were investigated using various spectroscopic analyses and molecular docking experiments.The results showed that binding of C3G with 7S and 11S at pH 2.0 and 5.0 not only reduced the hydrophobic environment around Tyr residues but also increased their polarity,while α-helical content increased and β-sheet content decreased in both 7S and 11S.Under pH 7.0 condition,the presence of C3G led to an increase in both α-helical and β-sheet content in 7S and 11S except for β-turns.Furthermore,the binding of C3G with 7S and 11S was an exothermic process,and at different pH conditions,hydrogen bonding and van der Waals forces were the main driving forces for the interaction between C3G and 7S/11S,resulting in static fluorescence quenching of 7S and 11S. Both 7S and 11S exhibited the highest affinity for C3G at pH 7.0. Molecular docking revealed that GLU229,ARG356,and PRO101 residues on 7S,and ARG161,VAL162,ILE171,and THR176 residues on 11S played key roles in the binding with C3G.Moreover,the binding of 7S and 11S with C3G significantly enhanced the oxidative stability and antioxidant capacity of C3G at different pH conditions.

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历史
  • 收稿日期:2023-08-15
  • 最后修改日期:2024-03-21
  • 录用日期:2024-03-22
  • 在线发布日期: 2024-03-22
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