(1.南昌大学 食品科学与技术国家重点实验室, 江西 南昌 330047;2.南昌大学 食品学院, 江西 南昌 330047;3.南昌大学 中德联合研究院, 江西 南昌 330047)
(1.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2.College of Food Science and Technology, Nanchang University, Nanchang 330047, China;3.Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China)
National Natural Science Foundation of China (32060584).
To study the effect of tropomyosin of Macrobrachium nipponense on macrophage polarization, the differential analysis of gene expression during tropomyosin-induced macrophage polarization was analyzed by transcriptomic analysis. The results showed that compared with the phosphate buffer saline group (PBS group), 69 genes were up-regulated and 154 genes were down-regulated in the tropomyosin-induced group (TM group), and 180 pathways were enriched. In the lipopolysaccharide and IFN-γ combined-induced M1 macrophage group(LPSIFN group), 1346 genes were up-regulated, 1360 genes were down-regulated, and 308 pathways were enriched. Compared with the IL-4-induced M2 macrophage group(IL4 group), 455 genes were up-regulated in the TM group, 446 genes were down-regulated and enriched to 269 pathways. According to the KEGG results, five signaling pathways that may be involved in macrophage polarization were selected, including NOD-like receptor signaling pathway, Jak-STAT signaling pathway, NF-κB signaling pathway, Toll-like receptor signaling pathway and PI3K/Akt signaling pathway. The expression of NOD2, TLR2, AKT3, NLRP3, Caspase-1, CD14, Lat, Myd88, NFKBIA and STAT1 genes in these five pathways were verified, and the expression levels of NOD2, TLR2, NLRP3, and STAT1 in the TM group were increased significantly. The NOD-like receptor signaling pathway, Jak-STAT signaling pathway, and Toll-like receptor signaling pathway related to these four genes were involved in the process of tropomyosin-induced macrophage polarization. The purpose of this study was to provide a theoretical basis for the further analysis of the relationship between macrophage polarization and food allergic relationships.