1.Rizhao Center for Disease Control and Prevention;2.Beijing Advanced Innovation Center for Food Nutrition and Human Health，Beijing Technology & Business University
The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)； the Science and Technology Program of the Beijing Municipal Education Commission
无花果果实皮薄多汁,采后易受微生物侵染。分离筛选采后贮藏期间无花果发病部位的病原真菌,结合形态学观察、真菌rDNA 内转录间隔区(internal transcribed spacer,ITS)序列分析、系 统发育树构建进行研究,并将分离得到的病原真菌回接至健康果实,健康果实出现与分离果实相同的病症。最终从无花果叶子和果实上分别分离到4 株丝状病原真菌,其中179# 为链格孢菌(Alternaria alternata)、180#为气味镰刀菌( Fusarium odoratissimum)、181# 为内生炭疽杆菌( Colletotrichum endophyticum)和182#为总枝状毛霉(Mucor racemosus),其中气味镰刀菌、内生炭疽杆菌和总枝状毛霉为无花果上未见报道的病原真菌。利用Biolog 微生物鉴定系统对4 株病原真菌进行碳源代谢指纹图谱分析,发现它们对60 种碳源具有相似的代谢特征,包括L-阿拉伯糖、D-纤维二糖和α-环式糊精等56 种可利用碳源以及N-乙酰-D-半乳糖胺、N-乙酰-β-D-甘露糖胺、L-海藻糖和D-乳酸甲酯4 种不可利用碳源。研究结果旨在为无花果病原真菌的生物学特征以及采后病害防治的研究提供参考依据。
Ficus carica Linn, a tender and juicy fruit, is susceptible to damage and infection caused by microorganisms. In this work, pathogenic fungi were isolated from infected Ficus carica fruit and leaves during post-harvest storage. Based on morphological, internal transcribed spacer (ITS) sequence analysis of fungal isolates enabled species delimitation and their position on the phylogenetic combined tree, four pathogenic fungi were isolated from infected fruit and leaf. All isolates may have caused similar symptoms of the disease in healthy Ficus carica fruit. Four strains were identified as Alternaria alternata (179#), Fusarium odoratissimum (180#), as Colletotrichum endophyticum (181#), and Mucor racemosus (182#), among which Fusarium odoratissimum , Colletotrichum endophyticum and Mucor racemosus were the first report of causing disease of Ficus carica fruit. According to biolog microbial identification system, the carbon metabolic fingerprinting showed that four strains had similar metabolic characteristics to 60 carbon sources, including 56 available carbon sources such as L-arabinose, D-fibrinose and α -cyclodextrin, and 4 unavailable carbon sources such as N-acetyl-D-galactosamine, N-acetyl-β -D-mannosamine, L-trehalose and D-methyl lactate. This study provides a preliminary reference for biological features and post-harvest control measures for Ficus carica fruit diseases.