The nutritional value of Portunus trituberculatus in two different sea areas was comprehensively evaluated by analyzing and comparing the muscle nutritional components. The water content, ash, crude protein, crude fat, fatty acid, amino acid, mineral element, nucleotide, betaine and cholesterol in muscle were determined by the national standard method. Nutritive quality evaluation was conducted according to the Food and Agricultural Organization (FAO) standards, and the correlation between nutrient composition, flavor content and production place was compared and analyzed. The results showed that there were significant differences in protein, amino acid, nucleotide, and betaine in two different sea areas. The contents of muscle protein, essential amino acids and total amino acids of wild male swimming crabs in Taizhou, Zhejiang were significantly higher than those in Rizhao, Shandong (P<0.05). The muscle contents of inosine acid and betaine of wild swimming crabs in Taizhou, Zhejiang were significantly higher than those in Rizhao, Shandong (P<0.05). There were some differences in muscle nutritional components and affected flavor and nutritional value of crabs from two different sea areas, which might be related to environmental factors, genetic factors and food intake.