外源茉莉酸甲酯和异亮氨酸对灰葡萄孢菌侵染后圣女果抗性相关酶酶活的影响
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四川轻化工大学

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四川轻化工大学研究生创新基金项目(y2019039)


Effects of Exogenous Methyl Jasmonate and Isoleucine on Cherry Tomatoes Resistance Related Enzyme Activity After Infection by Botrytis cinereal
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Sichuan University of Science and Engineering

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    摘要:

    果蔬在贮藏期间多发生灰霉病,造成果蔬严重腐烂。通过圣女果接种灰霉菌,模拟贮藏过程中可能发生的腐烂,研究外源茉莉酸甲酯(MeJA)、异亮氨酸(Ile)处理对抑制灰霉菌侵染的酶的影响。将Ile组和MeJA+Ile组用10mmol/L Ile浸泡处理后,对MeJA组和MeJA+Ile组用10μmol/L MeJA 30℃熏蒸48h,然后接种灰霉菌孢子侵染,室温贮藏并进行病斑直径和酶活测定。结果显示:MeJA、Ile处理的圣女果在灰霉菌侵染后苯丙氨酸解氨酶、多酚氧化酶、过氧化物氧化酶、超氧化物歧化酶、抗坏血酸过氧化物酶、过氧化氢酶酶活较高,与对照组差异显著(P<0.05)。MeJA+Ile处理组与其余3组比较,所测得的酶活均较高,而病斑直径较小,这说明MeJA和Ile联合处理的圣女果可提高抗病性酶活,增强灰霉菌侵染的抗性,对研究果蔬采后贮藏的灰霉菌腐烂防治有一定的意义。

    Abstract:

    During the storage period, more Botrytis cinerea occurred, which caused serious rot of fruits and vegetables .In this study, cherry tomatoes inoculation was used to simulate the rot that might occur in the storage process, and the inhibitory effect of exogenous methyl jasmonate (MeJA) and isoleucine (Ile) on the infection of gray mold was studied by enzyme activity.In the experiment, Ile group and MeJA+Ile group were first soaked in 10 mmol/L Ile, and the MeJA+Ile groups were fumigated with 10 μmol/L MeJA at 30℃ for 48h, and then inoculated with Botrytis cinerea spores infection, stored at room temperature, and the lesion diameter and enzyme activity were measured. The experimental results showed that cherry tomatoes treated by MeJA and Ile had higher enzyme activity in phenylalanineammonialyase、polyphenol oxidase、peroxidase 、superoxide dismutase、ascorbate peroxidase and catalase after infection, which was significantly different from that of the control group (P<0.05). Compared with the other three groups, the enzyme activity measured in the MeJA+Ile group in the experimental group was all higher, while the diameter of the disease spot was small, which indicated that cherry tomatoes treated by MeJA and Ile combined treatment could improve the activity of the disease resistance enzyme and enhance the resistance to the infection of Botrytis cinerea, which had certain research significance for the prevention and treatment of the rot of Botrytis cinerea in fruits and vegetables stored post-harvest.

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  • 收稿日期:2020-08-20
  • 最后修改日期:2021-01-26
  • 录用日期:2020-11-19
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