低共熔溶剂提取黄精多糖工艺优化及抗氧化活性研究
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Study on Optimal Process and Antioxidant Activity of Polygonatum sibiricum Polysaccharides Extracted by Deep Eutectic Solvents
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    摘要:

    选用不同离子组合的低共熔溶剂提取黄精多糖,筛选确定尿素-氯化胆碱低共熔溶剂提取黄精多糖效果最好,随后在单因素考察的基础上,采用响应面分析法优化尿素-氯化胆碱低共熔溶剂提取黄精多糖的工艺条件,并进行了低共熔溶剂重复利用对多糖提取率和含量的影响研究。通过测定总抗氧化能力、超氧阴离子(O-2)自由基和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率考察黄精多糖体外抗氧化活性。结果表明:低共熔溶剂提取黄精多糖的优化工艺参数为温度70℃、尿素-氯化胆碱物质的量比1.19、时间42.30min、液料比20.75mL/g,此条件下的多糖提取率为18.55%,较预测值相对误差为2.69%,比传统的热水浸提法增加了139.97%。低共熔溶剂重复利用不仅对黄精多糖提取率没有影响,在重复利用第3次时提取率最高,较新鲜溶剂提高了83.9%;而且黄精多糖含量随重复次数增加呈不断增加后趋于稳定的趋势,重复利用4、5次后多糖含量基本趋于稳定,达到63.48%、64.12%,说明其纯度不断提高。黄精多糖的O-2自由基清除能力、DPPH自由基清除能力、总抗氧化能力随质量浓度的增加而增加;在测定的质量浓度范围内,清除O-2自由基和DPPH自由基的IC50分别为0.524、0.951mg/mL,总抗氧化能力最高为241.2μg/mL。因此,低共熔溶剂法是一种效率高、清洁环保的新型黄精多糖提取方法。

    Abstract:

    In this study, Polygonatum sibiricum polysaccharides (PSPs) were extracted by deep eutectic solvents(DESs) with different ion combinations. The optimal effect of urea-choline chloride deep eutectic solvents was obtained. Based on single factor investigation, response surface analysis was used to investigate the optimum conditions for the extraction of PSPs by urea-choline chloride eutectic solvent. And the effect of DESs reuse on the extraction rate and content of PSPs was studied. The antioxidant activity of the PSPs in vitro was determined by measuring the total antioxidant capacity, O-2 free radicals and DPPH free radical scavenging rate. The results showed that the optimum process parameters for PSPs extraction by DESs were as follows:temperature 70℃, urea-choline chloride molar ratio 1.19, extraction time 42.30min, liquid to material ratio 20.75mL/g. Under this condition, the extraction rate of PSPs was 18.55%, and the relative error was 2.69% compared with the predicted value, which was 139.97% higher than that extracted by the traditional hot water. The DESs reuse not only had no effect on the extraction rate of PSPs, and the maximum was 83.9% higher than that of fresh solvents when reusing for the third time; and the content of PSPs increased continuously and tended to be stable with increasing the repeated times. After 4 or 5 times of reuse, the PSPs content was basically stabilized at 63.48% and 64.12%, respectively, which indicated that the purity was increasing. The total antioxidant capacity, scavenging rate of O-2 free radical and DPPH free radical was in dose dependent manner with mass concentration of PSPs, which was increased with the increase of mass concentration. The total internal antioxidant capacity was 241.2μg/mL, and the IC50 of O-2 free radicals and DPPH free radical scavenging capacity were 0.524mg/mL and 0.951mg/mL, respectively. Therefore, the DESs method was a new high-efficiency, clean and environment friendly method for extracting the Polygonatum sibiricum polysaccharides.

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汪涛,周新群,孙君社,张志民,王民敬,胡雪芳,裴海生.低共熔溶剂提取黄精多糖工艺优化及抗氧化活性研究[J].食品科学技术学报,2020,38(6):111-120.

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  • 收稿日期:2019-10-23
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  • 在线发布日期: 2020-12-28
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