(1.贵州大学 酿酒与食品工程学院, 贵州 贵阳 550025;2.贵州食品工程职业学院, 贵州 清镇 551400)
(1.School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;2.Guizhou Vocational College of Foodstuff Engineering, Qingzhen 551400, China)
To investigate the effects of characteristic ingredient vanillyl alcohol of Rhizoma gastrodiae on mycelial biomass and exopolysaccharide (EPS) synthesis, different mass concentrations of vanillyl alcohol were added to the Grifola frondosa fermentation system. The EPS production was determined by phenol-sulphuric acid method and the mycelial biomass was recorded. The results showed that a certain mass concentration of vanillyl alcohol could promote the biomass and EPS synthesis of Grifola frondosa. The optimal effect of vanillyl alcohol on the biomass and EPS synthesis of Grifola frondosa was shown at mass concentration of 200 mg/L, which were significantly increased by 21.1% and 19.9% compared to the blank control group (no addition of vanillyl alcohol), respectively (P<0.05). In addition, during the whole fermentation process with vanillyl alcohol, the fermentation began to stabilize on the 13th day, and the biomass and EPS yield were the maximum on the 14th day, which were(1.42±0.01)g/L and (2.10±0.03)g/L, respectively. However, they were both lower than that fermentation with Rhizoma gastrodiae alcohol extract or p-hydroxybenzaldehyde test group. On the 14th day, the content of vanillyl alcohol was decreased by 68.3% in comparison with that on the 0th day. As a characteristic ingredient in Rhizoma gastrodiae alcohol extract, vanillyl alcohol could be used for promoting the mycelial growth and EPS synthesis by Grifola frondosa.