天麻中香草醇对灰树花菌丝体生物量和胞外多糖合成的影响
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(1.贵州大学 酿酒与食品工程学院, 贵州 贵阳 550025;2.贵州食品工程职业学院, 贵州 清镇 551400)

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Effects of Vanillyl Alcohol of Rhizoma gastrodiae on Mycelial Biomass and Synthesis of Exopolysaccharide by Grifola frondosa
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(1.School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China;2.Guizhou Vocational College of Foodstuff Engineering, Qingzhen 551400, China)

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    摘要:

    为探讨天麻醇提物特征成分香草醇对灰树花菌丝体生物量和胞外多糖(exopolysaccharide, EPS)合成的影响,在灰树花发酵体系中添加不同质量浓度的香草醇,采用苯酚-硫酸法测定多糖含量并记录菌丝体质量。研究表明:一定质量浓度的香草醇对灰树花菌丝体生长和EPS合成有促进作用;香草醇质量浓度为200mg/L时,促进灰树花菌丝体生长和EPS合成的效果最佳,相比于空白组(未添加香草醇)分别增加了21.1%和19.9%,均显著高于空白组(P<0.05)。此外,在香草醇参与的灰树花整个发酵过程中,发酵第13天开始生物量和EPS产量趋于稳定,第14天时灰树花菌丝体生物量和EPS产量为最大值,分别为(1.42±0.01)g/L和(2.10±0.03)g/L,但低于天麻醇提物和对羟基苯甲醛实验组;且第14天同第0天相比,香草醇含量降低了68.3%。香草醇作为天麻醇提物中的特征成分可以被灰树花用于自身菌丝体生长和EPS的合成。

    Abstract:

    To investigate the effects of characteristic ingredient vanillyl alcohol of Rhizoma gastrodiae on mycelial biomass and exopolysaccharide (EPS) synthesis, different mass concentrations of vanillyl alcohol were added to the Grifola frondosa fermentation system. The EPS production was determined by phenol-sulphuric acid method and the mycelial biomass was recorded. The results showed that a certain mass concentration of vanillyl alcohol could promote the biomass and EPS synthesis of Grifola frondosa. The optimal effect of vanillyl alcohol on the biomass and EPS synthesis of Grifola frondosa was shown at mass concentration of 200 mg/L, which were significantly increased by 21.1% and 19.9% compared to the blank control group (no addition of vanillyl alcohol), respectively (P<0.05). In addition, during the whole fermentation process with vanillyl alcohol, the fermentation began to stabilize on the 13th day, and the biomass and EPS yield were the maximum on the 14th day, which were(1.42±0.01)g/L and (2.10±0.03)g/L, respectively. However, they were both lower than that fermentation with Rhizoma gastrodiae alcohol extract or p-hydroxybenzaldehyde test group. On the 14th day, the content of vanillyl alcohol was decreased by 68.3% in comparison with that on the 0th day. As a characteristic ingredient in Rhizoma gastrodiae alcohol extract, vanillyl alcohol could be used for promoting the mycelial growth and EPS synthesis by Grifola frondosa.

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张宗启,吴天祥,刘力萍.天麻中香草醇对灰树花菌丝体生物量和胞外多糖合成的影响[J].食品科学技术学报,2020,38(6):62-68.

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  • 收稿日期:2019-07-25
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  • 在线发布日期: 2020-12-28
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