α-琼胶酶OUC-GaJJ96的异源表达及酶学性质
作者:
作者单位:

(1.中国海洋大学 食品科学与工程学院, 山东 青岛 266003;2.青岛海洋科学与技术国家试点实验室/海洋药物与生物制品功能实验室, 山东 青岛 266237)

作者简介:

通讯作者:

中图分类号:

基金项目:


Heterologous Expression and Enzymatic Properties of αAgarase OUC-GaJJ96
Author:
Affiliation:

(1.College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;2.Qingdao National Pilot Laboratory for Marine Science and Technology/ Laboratory for Marine Drugs and Bioproducts, Qingdao 266237, China)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    琼胶酶在功能性琼胶寡糖的酶法制备中发挥着重要作用,目前有关α-琼胶酶的研究较少。克隆表达了来自溶藻性吉尔维菌(Gilvimarinus agarilyticus)的α-琼胶酶,命名为OUC-GaJJ96,并对其酶学性质及水解产物进行研究。该酶属于糖苷水解酶96家族,基因全长为3759bp,编码1252个氨基酸,N端含有26个氨基酸编码的信号肽,蛋白分子质量约为180kDa。酶学性质研究显示:OUC-GaJJ96最适反应温度和最适反应pH值分别为30℃和7.0(Tris-HCl缓冲液),比酶活力为2.68U/mg。通过高效液相色谱和电喷雾离子源质谱对OUC-GaJJ96的水解产物进行分析,结果表明:该酶水解产物是琼二糖、琼四糖和琼六糖,其中琼四糖是主要产物,OUC-GaJJ96可用于制备具有生物活性的琼胶寡糖。

    Abstract:

    agarase plays an important role in the enzymatic preparation of functional agaro-oligosaccharides. However, there are only few studies focused on α-agarases. An α-agarase named OUC-GaJJ96 from Gilvimarinus agarilyticus was cloned, and its enzymatic properties together with hydrolysis products were studied. OUC-GaJJ96 belong to glycoside hydrolase 96 family with a molecular weight of approximately 180 kDa. The gene OUC-GaJJ96 comprised a 3759bp, encoding a protein of 1252 amino acids, with a signal peptide of 26 amino acids at its N-terminal. The enzymatic properties of OUC-GaJJ96 showed that the optimal temperature and pH were 30℃ and 7.0 (Tris-HCl buffer), respectively. The specific activity of OUC-GaJJ96 was 2.68U/mg. The analysis of HPLC and ESI-MS demonstrated that the hydrolysis products of OUC-GaJJ96 were agarobiose, agarotetraose and agarohexaose, in which agarotetraose was the main product. Therefore, OUC-GaJJ96 has potential for the manufacture of bioactive agaro-oligosaccharides.

    参考文献
    相似文献
    引证文献
引用本文

金佳,江承程,毛相朝.α-琼胶酶OUC-GaJJ96的异源表达及酶学性质[J].食品科学技术学报,2020,38(6):47-54.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2020-11-02
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2020-12-28
  • 出版日期: