1.College of Life Science and Technology,Henan University of Urban Construction,Pingdingshan;2.College of Life Science and Technology,Henan University of Urban Construction,Pingdingshan,;3.China;4.State Key Laboratory of Food Science and Technology,Nanchang University
The optimum extraction conditions of polysaccharides from Chinese leek seed meal by using Polyethylene glycol/ammonium sulfate diaqueous system were investigated using the Response Surface Method (RSM) and the antioxidant activity of extract was obtained under optimum extraction conditions. The results indicated that the optimum extraction conditions were PEG molecular mass was 6000, PEG mass fraction was 14.50%, ammonium sulfate mass fraction was 20.46%. Under the optimal conditions, the extraction rate of polysaccharides from Chinese leek seed meal was 85.39%. Antioxidant activity of the polysaccharides from Chinese leek seed meal, including the DPPH., .OH and reducing power measure. The results showed that the leek polysaccharides possessed antioxidant properties in vitro. And with the concentration of polysaccharides increasing, its antioxidant activity gradually becomes stronger.