In this study, Polygonatum Sibiricum Polysaccharides were extracted from several deep eutectic solvents with different ion combinations. The best extraction effect of urea/choline chloride deep eutectic solvents was obtained. Then, based on single factor investigation, response surface analysis was used. The optimum conditions for the extraction of the polysaccharides from Polygonatum Sibiricum by urea/choline chloride eutectic solvent were investigated. The antioxidant activity of the polysaccharides in vitro was determined by measuring the total antioxidant capacity, O2-·free radicals and DPPH free radical scavenging rate. The results showed that the optimum process parameters for extracting the polysaccharides by deep eutectic solvents were temperature 70 ℃, urea/choline chloride molar ratio 1.19 mol/mol, time 42.30 min, liquid to material ratio 20.75 mL/g. This condition, the extraction rate is 18.55% of the polysaccharides, and the relative error is 2.69% compared with the predicted value, which is 139.97% higher than the traditional hot water extraction. The total antioxidant capacity, O2-·free radical and DPPH free radical scavenging rate of the polysaccharides were dose-effect relationship with mass concentration, and increased with the increase of mass concentration, which had certain antioxidant capacity, O2-·free radical and DPPH free radical scavenging ability. The total internal antioxidant capacity was 241.2 units/mL, and the IC50 values of O2-·free radicals and DPPH free radical scavenging capacity were 0.524 mg/mL and 0.951 mg/mL, respectively. Therefore, the deep eutectic solventsmethod is a new high-efficiency, clean and environment friendly method for extracting the polysaccharides.