精氨酸等物质对紫色红曲菌Monacolin K及红曲色素合成的影响
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1.北京工商大学;2.北京工商大学 北京市食品添加剂工程技术研究中心

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大学生科学研究与创业行动计划(G055);人才培养质量建设-一流专业建设(市级)-食品科学与工程(PXM2019_014213_000010);北京市科技新星计划(Z181100006218021);青年拔尖“十三五”时期北京市属高校高水平教师队伍建设支持计划(CIT&TCD201804023);北京市自然科学基金项目(KZ201810011015)


Effects of Arginine and Other Substances on Synthesis of Monacolin K and Monascus Pigment of Monascus purpureus
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Beijing Engineering and Technology Research Center of Food Additives,Beijing Laboratory of Food Quality and Safety,Beijing Technology and Business University BTBU

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    摘要:

    基于前期的非靶向代谢组学结果,通过在红曲菌发酵液原有营养成分的基础上添加精氨酸、L-苹果酸、D-葡萄糖、烟酰胺、α-酮戊二酸、苯丙氨酸、赖氨酸、焦磷酸硫胺素、黄素单核苷酸、L-乳酸等差异代谢物,对发酵第8天和第15天的红曲色素以及Monacolin K的产量进行了分析。结果表明:精氨酸、苯丙氨酸、赖氨酸以及黄素单核苷酸等物质的添加对Monacolin K产量的提高效果较好,推测氨基酸类物质的添加对Monacolin K产量有促进效果,其中精氨酸添加后效果最为显著,Monacolin K产量与对照组相比提高2.3~3.7倍。不同含量的烟酰胺对Monacolin K有不同的影响,低含量具有促进作用;而高含量则表现为抑制作用。赖氨酸、苯丙氨酸以及黄素单核苷酸对红曲色素提升效果较好,其中黄素单核苷酸可使红曲色素产量提高 1.5-3.0倍。

    Abstract:

    This study based on the preliminary results of the targeted metabonomics in Monascus purpureus,adding arginine, L-malic acid, D-glucose, niacinamide, α-oxoglutaric acid, phenylalanine, lysine, thiamine pyrophosphate,flavin mononucleotide and L-lactic acid as differential metabolites on the basis of the original nutrients, respectively. The yields of Monascus pigment and Monacolin K in 8th and 15th days were detected. The results showed that the addition of arginine, phenylalanine, lysine and flavin mononucleotide had the function of promotion on Monacolin K. It is speculated that the addition of amino acids could promote the yield of Monacolin K. Among them, the Monacolin K yield was 2.3 to 3.7 times higher than that of the control group when the addition of arginine. Different concentrations of niacinamide had different effects on Monacolin K. The lower had a simulative effect, while higher showed an inhibitory effect. Lysine, phenylalanine and flavin mononucleotide had a better effect on the enhancement of Monascus pigment, and flavin mononucleotide increased the yield by 1.5~3.0 times.

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  • 收稿日期:2019-10-08
  • 最后修改日期:2019-12-18
  • 录用日期:2019-11-18
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