The chemiluminescence technology was selected to establish a new method for the determination of sulfites in foods. Various factors affecting the chemiluminescence detection were evaluated and the optimum determination conditions were obtained in this study. The detection limit of the method was 4.6mg/kg. In the range of 1.0-10.0mg/L, the sulfite concentration was positively correlated with the luminous intensity value (ΔI), and the correlation coefficient (R²) was 0.9934. Spike recovery tests of six different samples were evaluated. The average RSD was 3.05% and the average recovery was 93.0%. Chemiluminescence determination results indicated that the new method was a good candidate for the qualitative and quantitative analysis of sulfites in foods.